• P-ISSN 0973-7200 E-ISSN 2454-8405
  • Follow us

Journal of Pharmaceutical Research


Journal of Pharmaceutical Research

Year: 2023, Volume: 22, Issue: 1, Pages: 21-26

Original Article

A Study of the Antihyperlipidemic and Antioxidant Activities of Ethanolic Extracts of Leaves of Houttuynia cordata Thunb . (EELHC) in Albino Rats


The present study was designed to evaluate the antihyperlipidemic and antioxidant activities of leaves of Houttuynia cordata Thunb. The ethanolic extract was obtained by percolation method and acute oral toxicity tests were performed according to OECD (Organization for Economic Cooperation and Development) guidelines. Hyperlipidaemia was induced by feeding the rats with a high-fat diet consisting of coconut oil and vanaspati ghee, in a ratio of 2:3 v/v at a dose of 10 ml/Kg body weight. The extract was given at a dose of 500mg/kg body weight. Lipid profile, Malondialdehyde (MDA), Catalase (CAT), and Superoxide Dismutase (SOD) were measured using standard methods. The extract showed a significant decrease in total cholesterol, triglycerides, LDL, and MDA in the blood. On the other hand, HDL, CAT, and SOD increased significantly. The study demonstrates that the ethanolic extract of leaves of Houttuynia cordata Thunb., decreases blood lipid levels and lipid peroxidation.


Keywords: Antihyperlipidemic, Antioxidant, Houttuynia cordata Thunb, EELHC


  1. Ross R. Atherosclerosis--an inflammatory diseaseN Engl J Med1999;340(2):115126. Available from: https://doi.org/10.1056/NEJM199901143400207
  2. Shrivastava R, Solanki SS, Tomar V, Garud N, Kannojia P. Comparative Evaluation of polyherbal combination for hypolipidemic activityIndian Journal of Pharmaceutical Sciences and Drug Research2009;1(1):912. Available from: https://doi.org/10.25004/IJPSDR.2009.010103
  3. Ashakumary L, Vijayammal PL. Additive effect of alcohol and nicotine on lipid metabolism in ratsIndian J Exp Biol1993;31(3):270274.
  4. Sudha SS, Karthic E, NaveenRengaramanujam J. Antihyperlipidaemic activity of Spirulinaplatensis in Triton x-100 induced hyperlipidaemic ratsHygeia: Journal for drugs and medicines2011;3(2):3237. Available from: http://www.hygeiajournal.com/Downloads/2337269785.SUDHA.pdf
  5. Pandya N, Santani D, Jain S. Antioxidant activity of ezetimibe in hypercholesterolemic ratsIndian Journal of Pharmacology2006;38(3):205206. Available from: https://doi.org/10.4103/0253-7613.25811
  6. Ikeda I, Sugano M. Inhibition of cholesterol absorption by plant sterols for mass interventionCurr Opin Lipidol1998;9(6):527531. Available from: https://doi.org/10.1097/00041433-199812000-00003
  7. Liang HX. On the evolution and distribution in SauruaceaeActa Bot Yunnan1995;17(3):13. Available from: https://journal.kib.ac.cn/EN/Y1995/V17/I03/1
  8. Ravindran PN. The Encyclopedia of Herbs and Spices. (Vol. 1 , pp. 233-237) Wallingford, UK. CAB International. 2017.
  9. Yoshinoh IN, Nabae K, Doi Y, Tamano S, Ogawa K, Shirai T. Thirteen-week oral toxicity study of Dokudami extract (Houttuynia cordata Thunb.) in F344/ DuCrj ratsJ Toxicol Pathol2005;18:175182. Available from: https://doi.org/10.1293/tox.18.175
  10. Lu HM, Liang YZ, Wu XJ, Qiu P. Tentative fingerprint-efficacy study of Houttuynia cordata injection in quality control of traditional Chinese medicineChem Pharm Bull (Tokyo)2006;54(5):725730. Available from: https://doi.org/10.1248/cpb.54.725
  11. Gennaro AR. Solution, Emulsion, Suspension, and Extracts. In: Remington: The Science and Practice of Pharmacy (19). (pp. 1495-1523) UK.  Lippincott Williams & Wilkins.. 2006.
  12. Ghosh MN. Fundamentals of Experimental Pharmacology (6). (pp. 15-18) Kolkata. Hilton and Company. 2015.
  13. Jain GC, Jhalani S, Agarwal S, Jain K. Hypolipidemic and Antiatherosclerotic Effect of Leptadenia pyrotechnica Extract in Cholesterol Fed RabbitsAsian J. Exp. Sci2007;21(1):115122. Available from: http://ajesjournal.com/PDFs/07-1/13-G%20C%20Jain.pdf
  14. National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III) Third report of the National Cholesterol Education Program (NCEP) Expert panel on detection, evaluation and treatment of high blood cholesterol in adults (adult treatment panel III) final reportCirculation2002;106(25):31433421. Available from: https://doi.org/10.1161/circ.106.25.3143
  15. Mahdi AA, Chandra A, Singh RK, Shukla S, Mishra LC, Ahmad S. Effect of herbal hypoglycemic agents on oxidative stress and antioxidant status in diabetic ratsIndian J Clin Biochem2003;18(2):815. Available from: https://doi.org/10.1007/BF02867361
  16. Fossati P, Prencipe L. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical Chemistry1982;28(10):20772080. Available from: https://pubmed.ncbi.nlm.nih.gov/6812986/
  17. Beers RF, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalaseJournal of Biological Chemistry1952;195(1):133140. Available from: https://pubmed.ncbi.nlm.nih.gov/14938361/
  18. Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutaseIndian J BiochemBiophys1984;21(2):133140. Available from: https://pubmed.ncbi.nlm.nih.gov/6490072/
  19. Visavadiya NP, Narasimhacharya A. Hypolipidaemic and antioxidant activity of Asparagus racemosus in hypercholesteraemic ratsIndian J Pharmacol2005;37(6):376380. Available from: https://www.ijp-online.com/text.asp?2005/37/6/376/19074
  20. Saikia H, Lama A. Effect of Leaves on Serum Lipids in Albino Rats Fed with High Fat DietInternational Journal of Pharmaceutical Sciences and Drug Research2011;3(2):141145. Available from: http://www.ijpsdr.com/index.php/ijpsdr/article/view/266
  21. Parasuraman S, Kumar EP, Kumar A, Emerson S. Antihyperlipidemic effect of Triglize, a polyherbal formulationInternational Journal of Pharmacy and Pharmaceutical Sciences2010;2(3):118122. Available from: https://innovareacademics.in/journal/ijpps/Vol2Issue3/607.pdf
  22. Lakhsmi BVS, Neelima N, Kasthuri N, Umarani V, Sudhakar M. Anti-hyperlipidaemic activity of Bauhunia purpura extracts in hypercholesterolaemic albino ratsInternational Journal of Pharmtech Research2011;3(3):12651272. Available from: https://sphinxsai.com/Vol.3No.3/pharm/pdf/PT=08(1265-1269)JS11.pdf
  23. Dhamija HK, Chauhan AS. Herbs: Now in arena of hyperlipidemiaDer Pharmacia Sinica2011;2(3):5159.
  24. Dash DK, Yeligar VC, Nayak SS, Ghosh T, Rajalingam R, Sengupta P, et al. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. on paracetamol-induced hepatotoxicity in ratsTropical Journal of Pharmaceutical Research2007;6(3):755765. Available from: https://doi.org/10.4314/tjpr.v6i3.14656
  25. Satishchandra A, Sumithra M. Synergistic effect of Mimusopselengiand Moringa oleifera onhigh-fat diet induced atheroma in ratsInternational Journal of Advances in Pharmaceutical Research2011;2(6):293300.
  26. Yu YMM, Wu CHH, Tseng YHH, Tsai CE, Chang WC. Antioxidative and Hypolipidemic Effects of Barley Leaf Essence in a Rabbit Model of AtherosclerosisJapanese Journal of Pharmacology2002;89(2):142148. Available from: https://doi.org/10.1254/jjp.89.142


© 2023 Published by Krupanidhi College of Pharmacy. This is an open-access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/


Subscribe now for latest articles and news.