Ankit Tyagi 1*, Nitin Sharma 1, Karan Mittal 1, Tilak Raj Bhardwaj 1, Arti Thakkar 2
- I. S. F. College of Pharmacy, Ferozepur Road, Moga � 142 001, Punjab, India
- Delhi Pharmaceutical Science and Research University, Govt. of NCT of Delhi, Pushp Vihar, Sec-3, New Delhi, 110017, India
Abstract
Purpose: The study was performed with the aim to design a Simple, precise and economical RP-HPLC method have been developed for simultaneous estimation of amitriptyline HCl and chlordiazepoxide in pharmaceutical formulation.Approach: RP-HPLC was developed using WATERS C18 250 mm � 4.6 mm (5 ?m) column, utilizing a mobile phase of methanol: acetonitrile: 0.2 M orthophosphoric acid, pH 4.5 (adjusted with triethylamine) 56:24:20 ml (with a flow rate of 1ml/min) and U.V detection was carried out at 240nm. Optimization of this method was carried out as a function of mobile phase composition, pH and flow rate. Peak purity was assessed by spectral comparison method.Findings: RP-HPLC method shows linearity in the range of for 0.05-1.6 ?g/ml and 0.01-0.32?g/ml amitriptyline HCl and chlordiazepoxide respectively. The LOD and LOQ values for amitriptyline HCl were 0.0250?g/ml and 0.0759 ?g/ml and 0.0045 ?g/ml and 0.0135 ?g/ml for chlordiazepoxide. The results of % purity was found to be 99.97 � 0.58 % w/w and 99.29 � 0.59 % w/w for HPLC method and 98.87 � 0.03 % w/w and 98.81 � 1.04 % w/w for first derivative spectrophotometric method for amitriptyline HCl and chlordiazepoxide respectively.Conclusion: Results of descriptive statistics studies conclude that RP-HPLC method found to be more sensitive and convenient than first derivative spectrophotometric method.
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