Mohammed Gulzar Ahmed 1*, A. Acharya 1, R. Chaudhari 1, K. Panicker 2, R. Reddy 2
- Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, B.G. Nagara-571448, Karnataka, India
- Department of Periodontology, KSR Institute of Dental Science and Research,Thiruchengodu - 637 215, Tamil Nadu, India
Abstract
Objective: The main objective of present study was to formulate and evaluate methyl cellulose based in situ gel of rosuvastatin for the treatment of periodontal diseases.Methods: The rosuvastatin in situ gel was prepared by using different concentration of methyl cellulose as gel base and gel was evaluated for pH, viscosity, rheology, drug content, syringeability, spreadability, drug release and drug release kinetics studies.Results: Compatibility study was performed using FT-IR and results showed there was no interaction between drug and other excipients. Viscosity of all formulations was found in the range of 320-590 centipoise and all formulations exhibited shear thinning pseudoplastic behaviour. Gelation time and temperature was found in the range of 2-15 min and 26�C-39�C respectively. All the formulation except formulation F6, F7 and G8 passed syringeabilitytest, as these formulations easily gets expelled from the syringe. An in vitro release study was conducted using 1.2 pH buffer for 8 hours and results showed that formulation F5 containing 0.9% methyl cellulose was considered as optimum formulation as it released 54.33% drug at the end 8 hours. In vitro release study revealed that release rate of drug from the in situ gel was concentration dependent; as concentration of methyl cellulose increased the drug release rate was retarded. Conclusion: Thus, it can be concluded that formulation F5 containing 0.9%w/v of methyl cellulose as gel base was considered as an optimized formulation, as it release drug in sustain manner in the treatment of periodontal diseases.
References
- Borole PM, Chaudhari YS, Dharashirakar SS, Kumavat SD, Shenghani K, Shah PR. Preparation and evaluation of in situ gel of Levofloxacin hemihydrate for treatment of periodontal disease. Int J Pharma Res Bio Sci. 2013; 2(3):185-96.
- Garala K, Joshi P, Shah M, Ramakrishna A, Patel J. Formulation and evaluation of periodontal in situ gel. Int J Pharm Invest. 2013; 3(1):2941.
- Patel KS, Vadalia KR, Patel JK. Development and evaluation of in situ gelling system for treatment of periodontitis. Der Phamacia Lettre. 2014; 6(4):124-31.
- Narayana RC, Harish NM, Ahmed MG, Prabhu P. Formulation and in vitro evaluation of in situ gels containing Secnidazole for vaginitis. Yakugaku Zasshi. 2009; 129(5):569-74.
- Sapra P, Patel D, Soniwala M, Chavda J. Formulation and optimization of in situ periodontal gel containing Levofloxacin for the treatment of periodontal diseases. J Sci Inno Res. 2013; 2(3): 607-26.
- �Q1A (R2): stability testing of new drug substances and products� in proceedings of the international Conference on hormonization (ICH'03), Geneva, Switzerland, 2003.
- Karavana SY, Rencber S, Senyigit ZA, Baloglu E. A new insitugel formulation of Itraconazole for vaginal administration. Pharamco Pharm. 2012; 3:417-26.
- Kaila HO, Ambasana MA, Thakkar RS, Saravaia HT, Shah AK. A new improved RP-HPLC method for assay of Rosuvastatin calcium in tablets. Indian J Pharm Sci. 2010; 72(5):592-598.
- Subramanian S. High dose of Atorvastatin reduces periodontal inflammation: A novel pleiotropic effect of statins. J Am Col Car 2013; 62(25): 2382-91.
- Harish NM, Prabhu P, Charyulu RN, Ahmed MG, Subrahmanyam EVS. Formulation and evaluation of in situ gels containing Clotrimazole for oral candidiasis. Indian J Pharm Sci. 2009; 71(4): 421-27.
- Kanpure VJ, Pande VV, Deshmukh PK. Dissolution enhancement of Rosuvastatin calcium by liquisolid compact technique. J Pharmaceutics. 2013; 13(2): 1-9.
