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Journal of Pharmaceutical Research

Article

Isolation and Characterisation of Chemical Constituents From Aerial Parts of Cardiospermum halicacabum
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Journal of Pharmaceutical Research

DOI: 10.18579/jopcr/v20.2.reshma

Year: 2021, Volume: 20, Issue: 2, Pages: 16-20

Original Article

Isolation and Characterisation of Chemical Constituents From Aerial Parts of Cardiospermum halicacabum

K Reshma1, RM Rohini1,∗

1Department of Pharmaceutical Chemistry, Krupanidhi College of Pharmacy, Bangalore, Karnataka, India

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Cardiospermum halicacabum or the "balloon vine" is a medicinal plant that belongs to the Sapindaceae family. The aim of this study was to isolate and identify its chemical constituents and derive insights into its pharmacological prospects. The aerial parts of Cardiospermum halicacabum were collected, washed, and dried at room temperature. The dried plant material was powdered and extracted using solvents such as petroleum ether, acetone, chloroform, and ethanol. The extractive values were calculated for each solvent. The extracts were analyzed by preliminary phytochemical screening to identify bioactive secondary metabolites. The active compounds were isolated by column chromatography and recrystallization methods. Thin-layer chromatography, high-performance thin-layer chromatography, high-performance liquid chromatography, and other spectroscopic methods have been used for compound identification. Phytochemical analysis confirmed the presence of saponins, phytosterols, triterpenoids, alkaloids, carbohydrates, proteins, and free amino acids in the extract. The isolated compound had a molecular formula of C39H56O2, a molecular weight of 557 g/mol, and a melting point of 145°C. Structural elucidation using IR, NMR, and MS confirmed that the compound was [(3S,4aR,6aR,6bS,8aR,11R,12S,12aR,14aR,14bR)-4,4,6a,6b,8a,11,12,14b-octamethyl-2,3,4a,5,6,7,8,9,10,11,12,12a,14,14a-tetradecahydro-1H-picen-3-yl] (E) 3-phenylprop-2-enoate. This research was able to isolate and identify a bioactive compound from Cardiospermum halicacabum, validating its chemical profile. The diverse array of chemical constituents suggests a significant pharmacological potential, supporting further exploration of this plant for medicinal applications.

Keywords: Cardiospermum halicacabum, HPLC, IR Spectra

References

  2. Raza SA, Hussain S, Riaz H, Mahmood S. Review of beneficial and remedial aspects of Cardiospermum halicacabum L. African Journal of Pharmacy and Pharmacology. 2013;7(48):3026–3033. Available from: https://academicjournals.org/article/article1389966050_Raza%20et%20al.pdf
  3. Jeyadevi R, Sivasudha T, Ilavarasi A, Thajuddin N. Chemical Constituents and Antimicrobial Activity of Indian Green Leafy Vegetable Cardiospermum halicacabum. Indian Journal of Microbiology. 2013;53(2):208–213. Available from: https://dx.doi.org/10.1007/s12088-012-0333-4
  4. Gulfraz M, Arshad M, Uzma N, Kanwal K, Shabir K. Comparison in Various Bioactive Compounds of Leaves and Seeds of Foeniculum Vulgare Mill. Ethnobotanical Leaflets. 2005;(9) 6–14. Available from: https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1265&context=ebl
  12. Boonmars T, Khunkitti W, Sithithaworn P, Fujimaki Y. In vitro antiparasitic activity of extracts of Cardiospermum halicacabum against third-stage larvae of Strongyloides stercoralis. Parasitology Research. 2005;97(5):417–419. Available from: https://dx.doi.org/10.1007/s00436-005-1470-z
  13. Waako P, Gumede B, Smith P, Folb P. The in vitro and in vivo antimalarial activity of Cardiospermum halicacabum L. and Momordica foetida Schumch. Et Thonn. Journal of Ethnopharmacology. 2005;99(1):137–143. Available from: https://dx.doi.org/10.1016/j.jep.2005.02.017
  14. Asha VV, Pushpangadan P. Antipyretic activity of Cardiospermum halicacabum. Indian Journal of Experimental Biology. 1999;37(4):411–414. Available from: https://pubmed.ncbi.nlm.nih.gov/10641181/
  15. Veeramani C, Pushpavalli G, Pugalendi KV. Antihyperglycaemic effect of Cardiospermum halicacabum Linn. leaf extract on STZ-induced diabetic rats. Journal of Applied Biomedicine. 2008;6:19–26. Available from: https://dx.doi.org/10.32725/jab.2008.003
  16. Grace XF, Shanthi S, Chamundeeswari D, Latha S, Seethalakshmi S, Manna P. Isolation and characterisation of Cardiospermum halicacabum mucilage. International Journal of Green Pharmacy. 2011;5(4). Available from: https://www.greenpharmacy.info/index.php/ijgp/article/view/220
  17. Patil AG, Joshi KA, Patil DA, Chandra N. Pharmacognostical standardization and HPTLC fingerprint of Cardiospermum halicacabum L. stem. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2011;2(2):343–352. Available from: https://www.rjpbcs.com/pdf/2011_2(2)/43.pdf
  18. Muthumani P, Meera R, Devi P, Mohamed SA, Arabath S, Koduri LVSK. Chemical investigation of Toddalia asiatica Lin. and Cardiospermum halicacabum Lin. International Journal of Drug Formulation and Research. 2010;1:224–239. Available from: https://www.researchgate.net/publication/284026903_Chemical_investigation_of_Toddalia_asiatica_Lin_and_Cardiospermum_halicacabum_Lin
  19. Murti K, Panchall MA, Lambole V, Gajera V. Pharmacological properties of cardiospermum halicacabum-A review. Pharmacologyonline. 2010;2:1005–1009. Available from: https://pharmacologyonline.silae.it/files/newsletter/2010/vol2/107.Murti.pdf

Copyright

© 2021 Published by Krupanidhi College of Pharmacy. This is an open-access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/) 

  • 04 June 2025

Year: 2021, Volume: 20, Issue: 2


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