Raja sundararajan *, Christopher Vasanth Kumar , Jayaveera
- GITAM Institute of Pharmacy, GITAM University, Visakhapatnam- 530 045, Andhra Pradesh, India
Abstract
Purpose: The present work describes analytical method development and validation for the estimation of Hydrochlorothiazide in different combinations using UPLC. Methodology: The separation was achieved on column ACE C18 (2? x 3mm x 50mm), using mobile phases method A, B, C (0.5% Ammonium Acetate buffer solution: Methanol (50:50v/v)), D, E (0.005M KH2PO4 Buffer: Methanol: ACN (40:52:8) final PH-3 with orthophosporic acid.) respectively. The retention time of Hydrochlorothiazide were found to be 0.59, 0.60, 0.58, 0.61, 0.69 mins for method A, B, C, D, E, respectively with combination. Finding: The method was validated as per ICH, for Accuracy, Precision, Linearity and Robustness, specisity, LOD and LOQ. Conclusion: The developed and validated method was used to estimated the Hydrochlorothiazide in its different combinations. In all combinations % of assay were same, this method was used to routine quality control analysis.
References
- www.wikipedia.org/wiki/Losartan Potassium
- www.wikipedia.org/wiki/Hydrochlorothiazide
- Ramana Rao G, Murthy SSN, Khadgapathi P. High performance liquid chromatography and its role in pharmaceutical analysis. Eastern Pharmacist, 29(346), 1986, 53.
- Snyder LR, Kirkland JJ, Glajch JL. Practical HPLC method development. 2nd ed. New York: Wiley InterScience; 1997, 1-57.
- Byyny RL. Losartan potassium lowers blood pressure measured by ambulatory blood pressure monitoring. J. Hypertension, 13, 1995, S29-S33.
- Lastra OC, Lemus IG, Sanchez HJ, Perez RF. Development and validation of an UV derivative spectrophotometric determination of Losartan potassium in tablets. J Pharm Biomed Anal., 33, 2003, 175-80.
- Zarapkar SS and Kanyawar NS. Simultaneous estimation of Amlodipine and Losartan potassium in pharmaceutical dosage by RP-HPLC. Indian drugs, 39(6), 2002, 338-341.
- Deanne L Hertzog, et al. Developed and validated stabilityindicating HPLC method for the simultaneous determination of losartan potassium, hydrochlorothiazide, and their degradation products. Pharmacopoeial Forum, 31(5), 2005, 1453-1463.
- Wang ZY, Tain, Tain XL, Tain T. Simultaneous determination of Valsartan and Hydrochlorothiazide in tablets by RP-HPLC. Indian journal of pharmaceutical sciences, 70(3), 2008, 372-374.
- Shah NJ, Suhagia BN, Shah RR, Shah PB. Development and validation of a HPTLC method for the simultaneous estimation of telmisartan and hydrochlorothiazide in tablet. Indian Journal of Pharmaceutical Sciences, 69(2), 2007, 202-205.
- Garg G, Saraf S. Spectrophotometric and column highperformance liquid chromatographic methods for simultaneous estimation of metoprolol tartrate and hydrochlorothiazide in tablets. JAOAC Int. 2008?91:1045�50.
- Gupta KR, Tajne MR, Wadodkar SG. New Spectrophotometric method for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in tablets. Indian J Pharm Sci. 2008?70:511�3.
- Bilal YILMAZ, Determination of Ramipril in pharmaceutical preparations by High?performance liquid chromatography. International Journal of Pharmaceutical Sciences Review and Research, Volume 1, Issue 1, March � April 2010; Article 008, pp 39?42.
- Rao KV Kanna, Rao ME, Nagoji KE, Rao SS. Determination of Metoprolol Tartrate by reverse phase HPLC. Indian J Pharm Sci. 2003?65:204�6.
- Badulescu M, Balala UD, Cacovean I, Ilie M, Baconi DL. UVVisible spectrophotometeric assay of metoprolol. Note2. Method validation. Farmacia. 2008?LVI(4):363�70.
- Sunil Jawla, K Jeyalakshmi, T Krishnamurthy, Y. Kumar Development and Validation of Simultaneous HPLC method for Estimation of Telmisartan and Ramipril in Pharmaceutical Formulations. Int.J. PharmTech Res.2010, Vol.2, No.2, pp 1625? 1633.
- M. M. Baing, V. V.Vaidya , R. T. Sane, S. N. Menon, K. Dalvi; Simultaneous RP?LC Determination of Losartan Potassium, Ramipril, and Hydrochlorothiazide in Pharmaceutical Preparations, Chromatographia 2006, 64, pp 293�296 September (No. 5/6)
- Tambe, Vijaya Vichare, Ujjawala Kandekar And Shashikant Dhole, Novel UV Spectrophotometric Methods For Estimation of Ramipril and Hydrochlorothiazide by Simultaneous Equation And Area Under Curve Method, Int J Appl Pharm , Vol 2, Issue 4, 20?22.
- Bankey, G. G Tapadiya, S. S Saboo, S. Bindaiya, Deepti Jain, S. S. Khadbadi; Simultaneous Determination of Ramipril, Hydrochlorothizide and Telmisartan by Spectrophotometry. Int.J. ChemTech Res.2009, Vol.1, No.2, pp 183?188.
- Budawari S. The Merck Index. Whitehouse Station, NJ: Merck and Co. Inc.; 2006. p. 927, 1573.
- Satana E, Altinay S, Goger NG, Ozkan SA, Senturk Z. Simultaneous determination of Valsartan and Hydrochlorothiazide in tablets by first order UV spectrophotometry and Liquid chromatography. J Pharm Biomed Anal 2001?25:100913
- Lande NR, Shektar BM, Kadam SS, Dhaneshwar SR. Simultaneous spectrophotometric estimation of Losartan potassium and Hydrochlorothiazide in tablet dasage form. Indian drugs 2000?37:57781.
- Dinc E, Ustundag O. A new application of chemometric technique to HPLC data for the simultaneous analysis of two compound mixture. J Liq Chrmatogr Relat Technol 2005?28:217994.
- Ulu ST, Saglik S. Comparison of UV and Second derivative spectrophotometric and HPLC methods for the determination of Losartan in tablets. Turk J Pharm Sci 2004?1:16575.
- Dinc E, Ustundag O. Application of multivariate calibration techniques to HPLC data for quantitative analysis of binary mixture of Hydrochlorothiazide and Losartan in tablet. Chromatographia 2005?61:23744.
- DL, Mc Cafferty JF, Fang XG, Tyrell RJ, Reed RA. Development and validation of stability indicating HPLC method for simultaneous determination of Losartan Potassium, Hydrochlorothiazide and their degradation product. J Pharm Biomed Anal 2002?30:74760.
