P. K. Saini 1*, C. L. Jain 2, R. M. Singh 1, S. C. Mathur 1, G. N. Singh 1
- Research and Development Division, Central Indian Pharmacopoeia Laboratory, Indian Pharmacopoeia Commission, Govt. of India, Ministry of Health & FamilyWelfare, Sector-23, Rajnagar, Ghaziabad-201002, India
- Department of Chemistry, M. M. H. College, Ghaziabad (U.P.), India
Abstract
A high-performance liquid chromatographic (HPLC) method has been developed for the determination of Artemisinin in Artemisia annua Herb. The HPLC separation was carried out in isocratic mode using C18, sunfire column (4.6 � 250 mm, 5 ?m particle size) with a mobile phase composed of acetonitrile : water (85:15 v/v) at a flow rate of 1.0 ml/min. The detection was monitored at 216 nm. The method validation parameters showed good results for linearity, precision, accuracy, specificity, and in recovery studies. The calibration curve for Artemisinin was found linear from the range of 500 to 1200 ?g/ml. The interday and intraday studies (relative standard deviation) was obtained within the limits. The proposed HPLC method is precise, accurate and rapid for determination of Artemisinin in Artemisia annua Herb.
