• P-ISSN 0973-7200 E-ISSN 2454-8405
  • Follow us

Journal of Pharmaceutical Research

Article

Journal of Pharmaceutical Research

Year: 2021, Volume: 20, Issue: 2, Pages: 6-10

Original Article

Isolation and Characterization of Chemical Constituents from Alangium Salvifolium Leaves Extract

Abstract

Alangium salvifolium is a medicinal plant that has been studied for its medicinal properties. This study aimed to isolate and identify bioactive molecules from A. salvifolium leaves and examine their medicinal properties. Dried leaves of A. salvifolium were extracted with petroleum ether, chloroform, and methanol using successive extraction protocols. The petroleum ether extract was then purified by column chromatography using a solvent gradient, petroleum ether, ethyl acetate, methanol, and silica gel. Isolated compound AS-01 was then analyzed by thin-layer chromatography, high-performance liquid chromatography, high-performance thin-layer chromatography, and different spectroscopic analyses, such as Fourier Transform Infrared, 1H-Nuclear Magnetic Resonance spectroscopy, and mass spectrometry. AS-01 was isolated as a white crystalline compound with a molecular weight of 528 and purity of 90%, as confirmed by HPLC. The compound showed strong structural characteristics in the form of hydroxyl, methoxy, and aromatic groups. The compound was positive for alkaloids, and the retention factor (Rf) value was aligned with the standards in both TLC and HPTLC. This study reports a novel alkaloid AS-01. The isolation and structural elucidation of AS-01 revealed new insights into the pharmacological potential of the plant.

Keywords: Alangium salvifolium, Alkaloids, Isolation, Column Chromatography, Spectroscopic Analysis

References

  1. Ratra M, Gupta R. Comprehensive Review on Pharmacological Profile of Alangium salvifolium: A Medicinal Plant. Pharmaceutical and Biosciences Journal. 2015;3(3):22–28. Available from: https://dx.doi.org/10.20510/ukjpb/3/i3/89396
  2. Gulfraz M, Arshad M, Uzma N, Shabir K. Comparison in various bioactive compounds of leaves and seeds of Foeniculum vulgare Mill. Ethnobotanical Leaflets. 2005;9:6–14. Available from: https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1265&context=ebl
  3. Kumar R, Pate DK, Prasad SK, Sairam K, Hemalatha S. Antidiabetic activity of alcoholic leaves extract of Alangium lamarckii Thwaites on streptozotocin–nicotinamide induced type 2 diabetic rats. Asian Pacific Journal of Tropical Medicine. 2011;4(11):904–909. Available from: https://dx.doi.org/10.1016/s1995-7645(11)60216-2
  4. Zahan R, Nahar L, Ne ML. Antinociceptive and Anti-inflammatory Activities of Flower (Alangium salvifolium) Extract. Pakistan Journal of Biological Sciences. 2013;16(19):1040–1045. Available from: https://dx.doi.org/10.3923/pjbs.2013.1040.1045
  5. Ahad HA, Padmaja BS, Sravanthi M, Ramyasree P, Kavitha K. Phytochemical screening and anti-inflammatory actions of Alangium salviifolium root extract. Natural Product Research. 2012;26(17):1649–1653. Available from: https://dx.doi.org/10.1080/14786419.2011.589054
  6. Jubie S, Jawahar N, Koshy R, Gowramma B, Murugan V, Suresh B. Anti-arthritic activity of bark extracts of Alangium salviifolium Wang. Rasayan Journal of Chemistry. 2008;1(3):433–436. Available from: https://rasayanjournal.co.in/vol-1/issue-3/2.pdf
  7. Rajamanickam V, Rajasekaran A, Quine S, Jesupillai M, Sabitha R. Diuretic activity of Alangium salvifolium subsp. hexapetalum. International Journal of Alternative Medicine. 2008;8(1). Available from: https://ispub.com/IJAM/8/1/5184#:~:text=The%20preliminary%20phytochemical%20analysis%20showed,in%20urine%20%5BTable%201%5D.
  8. Venkateshwarlu R, Raju AB, Yerragunta VG. Phytochemistry and pharmacology of Alangium salvifolium: A review. Journal of Pharmacy Research. 2011;4(5):1423–1425. Available from: https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=6d0615f582e473548bd2e889180670cc359e0ec3
  9. Jain S, Sinha A, Bhakuni DS. The biosynthesis of β-carboline and quinolizidine alkaloids of Alangium lamarckii. Phytochemistry. 2002;60(8):853–859. Available from: https://dx.doi.org/10.1016/s0031-9422(02)00057-2
  10. Tanwer BS, Vijayvergia R. Phytochemical evaluation and quantification of primary metabolites of Alangium salvifolium. International Journal of Pharmaceutical and Biological Sciences. 2010;1(3):1–6. Available from: https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=9773815bafb022965ea52181dfa3921399b79321
  11. Sharma AK, Agarwal V, Kumar R, Balasubramaniam A, Mishra A, Gupta R. Pharmacological studies on seeds of Alangium salvifolium Linn. Acta Poloniae Pharmaceutica - Drug Research. 2011;68(6):897–904. Available from: https://ptfarm.pl/pub/File/Acta_Poloniae/2011/6/897.pdf
  12. Ahad HA, Padmaja BS, Yesupadam P, Guruprakash P, Sravanthi M, Ramyasree P. Phytochemical and Hypoglycemic Evaluation of <i>Alangium Salviifolium</i> Root Extract. Journal of Scientific Research. 2011;3(2):393–402. Available from: https://dx.doi.org/10.3329/jsr.v3i2.7047

Copyright

© 2021 Published by Krupanidhi College of Pharmacy. This is an open-access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/

DON'T MISS OUT!

Subscribe now for latest articles and news.