Karthika Ashok Kumar 1*, Akhila Sivadas 2, A. S. Ananya Dutt 1, Chanshi Chandran 1, M. V. Thampi 3, K. Pavithran 4
- Amrita School of Pharmacy, Kochi, India
- Department of Oncology, Amrita Institute of Medical Sciences, Kochi, India
- Department of Pathology, Amrita Institute of Medical Sciences, Kochi, India
- Department Medical Oncology & Hematology, Amrita Institute of Medical Sciences, Kochi, India
Abstract
The discovery of 1p and 19q chromosomal arms deletion in glial tumors influences both more objective diagnosis and more accurate prediction of chemotherapy response.As a result an attempt has been made to detect deletion using fluorescence in-situ hybridization (FISH) and analyzed its prognostic value in a cohort of glial tumor patients from Amrita Institute of Medical Sciences and Research Center Kochi.FISH was performed on 66 FFPE tissue sections by using Vyis LSI 1p36/LSI 1q25 and LSI 19p13/LSI 19q13dual coloured FISH probe sets. Signals were scored from at least 150-250 non-overlapping, intact nuclei. 163 cases were analyzed. Both 1p and 19q deletions was observed only in 28/163 (17.17%), - 1p/+ 19q deletion 80/163 (49.07%) and+1p/-19q deletion 55/163(33.74%) .In this work presented the FISH was successfully applied to identify deletion 1p/19q. Its incidence depends on the type of diagnosed gliomas. In contrast to reported data, the present study reveals 49.07% deletion - 1p/ + 19q.Deletions also have prognostic significance in the test group what constitutes the basis for inclusion of determining deletion 1 p/19q into diagnostic and treatment algorithm.
