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Journal of Pharmaceutical Research

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Scientific Standardization of Various Extracts of Chenopodium giganteum D. Leaves
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Journal of Pharmaceutical Research

DOI: 10.18579/jopcr/v21i2.3

Year: 2022, Volume: 21, Issue: 2, Pages: 48-54

Original Article

Scientific Standardization of Various Extracts of Chenopodium giganteum D. Leaves

Shailin Dkhar​ ✉ 1 , E Akila 1 , V B Narayana Swamy 1 , N Pruthvi 1

1 Department of Pharmacognosy, R R College of Pharmacy, Bangalore­, Karnataka, 560090, India.

Corresponding author ✉: [email protected]

 

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

In the present research, the Histological examination, physiological evaluation, phytochemical screening, total phenolic content and total flavonoid content and TLC of leaf extracts of Chenopodium giganteum have been studied. Ethanol, aqueous, chloroform, and pet ether extracts of Chenopodium giganteum was prepared. Phytochemical screening shows presence of carbohydrates, proteins, amino acids, glycosides like saponin and flavonoids, tannins, phenols, alkaloids, and steroids. The physiological evaluation shows total ash 15.12% w/w and acid insoluble ash value 7.46%w/w, loss of drying was found to be 8.1% w/w, extractive value of pet ether extract was found to be 2.3% w/w, chloroform extract 4.5% w/w, ethanol extract 7.2% w/w and water 10.2% w/w. The total flavonoid content of pet ether extract was found to be 17.2227±0.0729 µg/ml, chloroform extract 23.7224±0.0878 µg/ml, ethanol extract 49.8601±0.0303 µg/ml and water extract 64.7705±0.0375 µg/ml. The total phenolic content of pet ether extract was found to be 14.477±0.0226 µg/ml, chloroform extract 17.764±0.0216 µg/ml, ethanol extract 19.518±0.0173 µg/ml and water extract 27.686±0.0233 µg/ml. TLC of pet ether extract shows constituents having Rf values 0.548, 0.274, 0.1935, and 0.080, chloroform extract shows constituents having Rf values 0.765, 0.656, 0.468, 0.234, and 0.156, ethanol extract shows constituents having Rf values 0.815 and 0.584, and water extract shows constituents having Rf values 0.704 and 0.064.

 

Keywords: Histological examination, physiological evaluation, phytochemical screening, Chenopodium giganteum

References

  1. Agarwal S. Clinically useful herbal drugs. (pp. 10-12) 2005.
  2. Sharma A, Shanker C, Tyagi LK, Singh M, Rao CV. Herbal medicine for market potential in India: an overview. Acad J Plant Sci. 2008;1(2):26–36. Available from: http://www.idosi.org/ajps/1%282%2908/2.pdf
  3. ZG, MS, ECS. Chenopodiaceae (Flora of China). in Flora of China. In: Zhengyi W, Raven PH., eds. Chenopodiaceae. (Vol. 5, pp. 351-414) Missouri Botanical Garden Press. 2003.
  4. Yadav N, Vasudeva N, Singh S, Sharma SK. Medicinal properties of genus Chenopodium Linn. Natural Product Radiance. 2007;6(2):131–134. Available from: http://nopr.niscpr.res.in/bitstream/123456789/7849/1/NPR%206%282%29%20131-134.pdf
  7. Panda H. Handbook on Medicinal Herbs with Uses. (pp. 325-326) New Delhi. Asia Pacific Business Press. 2005.
  8. Pramila K, Neetu S, Anju R. Medicinal plants used in traditional health care system prevalent in Western Himalaya. Indian J Traditional Knowle. 2006;5(3):300–309.
  9. Patwardhan B, Vaidya M, Chorghade. Ayurveda and natural products drug discovery. Current Science. 2004;86(6):789–799. Available from: https://www.jstor.org/stable/24109136
  10. Bhargava A, Shukla S, Ohri D. Evaluation of foliage yield and leaf quality traits in Chenopodium spp. Euphytica. 2007;153(1):199–213. Available from: https://doi.org/10.1007/s10681-006-9255-8
  11. Ishii Y, Takiyama K. Characterization of oxalic acid in vegetables. Analytical sciences. 1991;7:811–815.
  12. Wang Z, Ando A, Takeuchi A, Ueda H. Effects of cooking conditions on the relationships among oxalate, nitrate, and lutein in spinach. Food Science and Technology Research. 2018;24(3):421–426. Available from: https://doi.org/10.3136/fstr.24.421
  14. Bhargava A, Shukla S, Srivastava J, Singh N, Ohri D. Chenopodium: a prospective plant for phytoextraction. Acta Physiologiae Plantarum. 2008;30(1):111–120. Available from: https://doi.org/10.1007/s11738-007-0097-3
  15. Al-Saleh GF, El-Din AYG, Abbas JA, Saeed NA. Phytochemical and biological studies of medicinal plants in Bahrain: the family Chenopodiaceae-part 2. International journal of pharmacognosy. 1997;35(1):38–42. Available from: https://doi.org/10.1076/phbi.35.1.38.13266
  16. Mroczek A. Phytochemistry and bioactivity of triterpene saponins from Amaranthaceae family. Phytochemistry Reviews. 2015;14(4):577–605. Available from: https://doi.org/10.1007/s11101-015-9394-4
  17. Siener R, Hönow R, Seidler A, Voss S, Hesse A. Oxalate contents of species of the Polygonaceae, Amaranthaceae and Chenopodiaceae families. Food Chemistry. 2006;98(2):220–224. Available from: http://dx.doi.org/10.1016/j.foodchem.2005.05.059
  19. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. (pp. 181-184) 2001.
  22. Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies. 2020;8(2):603–611. Available from: https://doi.org/10.22271/chemi.2020.v8.i2i.8834
  23. Khandelwal K. Practical pharmacognosy. 2008.
  24. Pandey MK, Kumar A, Singh R, Tripathi M. Scientific standardization of leaves of Chenopodium album L. Journal of Pharmacognosy and Phytochemistry. 2016;5(5):1–6. Available from: https://www.phytojournal.com/archives/2016/vol5issue5/PartA/5-4-6-331.pdf
  25. Mathur R, Vijayvergia R. Determination of total flavonoid and phenol content in Mimusopselengi Linn. International Journal of Pharmaceutical Sciences and Research. 2017;8(12):5282–5285.
  26. Arora SK, Itankar PR, Yende SR. Phytochemical screening and TLC studies of different extracts of Chenopodium album. Journal of Ayurvedic and Herbal Medicine. 2020;6(1):15–20. Available from: http://www.ayurvedjournal.com/JAHM_202061_05.pdf
  27. Sathya R, Kanaga N, Sankar P, Jeeva S. 2017.

Copyright

© 2022 Published by Krupanidhi Educational Trust. This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/)

  • 27 September 2022

Year: 2022, Volume: 21, Issue: 2


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